Human stem cell technology and biology: a research guide and laboratory manual / ed. by G.S.Stein et al. - Hoboken: Wiley-Blackwell, 2011. - xvii, 419 p.: ill. + CD-ROM. - Incl. bibl. ref. - Ind.: p.413-419. - ISBN 978-0-470-59545-9  Место хранения:   02 | Отделение ГПНТБ СО РАН | Новосибирск

Оглавление / Contents

Foreword ....................................................... ix Preface ........................................................ xi Acknowledgments .............................................. xiii Editors and Contributors ....................................... xv SECTION I INTRODUCTION 1 Introduction to Pluripotent Stem Cells: Biology and Applications ................................................. 3 Maria Borowski and Gary S. Stein 2 Researching and Obtaining Established Stem Cell Lines ........ 9 Mai X. Luong, Kelly P. Smith, and Gary S. Stein SECTION II LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: PLURIPOTENT STEM CELL CULTURE 3 Basics of Cell Culture ...................................... 19 Alicia Allaire, Mai X. Luong, and Kelly P. Smith 4 The Stem Cell Laboratory .................................... 33 Alicia Allaire, Mai X. Luong, and Kelly P. Smith 5 Reagent Preparation ......................................... 41 Alicia Allaire, Mai X. Luong, and Kelly P. Smith 6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells .................................. 59 Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel 7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts ... 87 Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel 8 Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells ........................................ 95 Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski 9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates .......................... 105 Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski 10 Harvesting Human Embryonic Stem Cells for Cryopreservation ........................................... 119 Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski 11 Human Embryonic Stem Cell Culture on BD Matrigel™ with mTeSR®1 Medium ............................................. 129 Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski SECTION III LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: CHARACTERIZATION OF PLURIPOTENT STEM CELLS 12 Defining Pluripotency ...................................... 141 Kelly P. Smith and Mai X. Luong 13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy .............................. 149 Shirwin M, Pockwinse and Prachi N. Ghule 14 Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry .................................................. 159 Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski 15 Characterization of Human Embryonic Stem Cell Gene Expression Using Reverse Transcription Polymerase Chain Reaction ................................................... 167 Alicia Allaire, Kelly P. Smith, and Mai X. Luong 16 Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body Formation .................................... 191 Shirwin M. Pockwinse and Prachi N. Ghule 17 Characterization of Human Embryonic Stem Cells by Cytogenetics: Karyotyping and Fluorescence In Situ Hybridization .............................................. 199 Shirwin M. Pockwinse, Prachi N. Ghule and Anne Higgins 18 High-Resolution Chromatin Immunoprecipitation Assay ........ 211 Beatriz Pérez-Cadahia, Bojan Drobic, and James R. Davie 19 Assaying Pluripotency Via Teratoma Formation ............... 217 M. William Lensch and Tan A. Ince SECTION IV PERSPECTIVES IN HUMAN STEM CELL TECHNOLOGIES 20 Genomic Analysis of Pluripotent Stem Cells ................. 237 David Lapointe 21 Proteomic Analysis of Human Pluripotent Cells .............. 245 Andy T.Y. Lau, Yan-Ming Xu, and Jen-Fu Chiu 22 Bioinformatics Strategies for Understanding Gene Expression in Human Pluripotent Cells ...................... 257 Gustavo Glusman, Bruz Marzolf, Kai Wang, Ji-Hoon Cho, Burak Kutlu, and Qiang Tian 23 Epigenetic Analysis of Pluripotent Cells ................... 273 Mojgan Rastegar, Geneviève P. Delcuve, and James R. Davie 24 Differentiation of Pluripotent Stem Cells: An Overview ..... 289 Jeremy Micah Crook 25 Cellular Reprogramming: Current Technology, Perspectives, and Generation of Induced Pluripotent Cells ...................................................... 297 Tanja Dominko SECTION V APPLICATIONS OF HUMAN EMBRYONIC STEM CELLS 26 Human Pluripotent Cells: The Biology of Pluripotency ....... 313 Li-Fang Chu and Thomas P. Zwaka 27 Human Pluripotent Cells for Regenerative Medicine: Potential Applications for Regenerative Medicine ........... 327 Christopher C. Ford and Darreil N. Kotton 28 Therapeutic Applications of Human Embryonic Stem Cells ..... 339 Shi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas, and Robert Lanza 29 Human Stem Cells for Drug Screening, Toxicity, Specificity, and Off-Target Effects ........................ 353 Arnaud Lacoste and Mark Burcin 30 Interfacing Stem Cells with Gene Therapy ................... 367 Christian Mueller and Terence R. Flotte 31 Tissue Engineering for Stem Cell Mediated Regenerative Medicine ................................................... 377 Janet Zoldan, Thomas P. Kraehenbuehl, Abigail K.R. Lytton-Jean, Robert S. Langer, and Daniel G. Anderson Glossary ...................................................... 401 Index ......................................................... 413