Preface to first edition ...................................... vii
Preface to second edition ...................................... ix
Chapter 1. The tools for the job ............................... 1
Chapter 2. Polymerase chain reaction .......................... 30
Chapter 3. Simple cloning ..................................... 52
Chapter 4. Other vector systems for E. coli ................... 74
Chapter 5. Making libraries ................................... 98
Chapter 6. Screening libraries ............................... 116
Chapter 7. Modification and mutagenesis ...................... 143
Chapter 8. Use of cloned DNA ................................. 162
Chapter 9. Using other organisms ............................. 182
Chapter 10. Examples .......................................... 243
References .................................................... 250
Index ......................................................... 257
|