Preface ........................................................ ix
Acknowledgments ................................................ xi
Introduction ................................................. xiii
I Overview: Bridging Nonliving and Living Matter ............. 1
1 The Early History of Protocells: The Search for the Recipe
of Life ...................................................... 3
Martin M. Hanczyc
2 Experimental Approaches to Fabricating Artificial Cellular
Life ........................................................ 19
David Deamer
3 Semisynthetic Minimal Cells: New Advancements and
Perspectives ................................................ 39
Pasquale Stano, Giovanni Murtas, and Pier Luigi Luisi
4 A Roadmap to Protocells ..................................... 71
Steen Rasmussen, Mark A. Bedau, John S. McCaskill, and
Norman H. Packard
II Integration ............................................... 101
5 Steps Toward a Synthetic Protocell ......................... 107
Martin M. Hanczyc, Irene A. Chen, Peter Sazani, and
Jack W. Szostak
6 Assembly of a Minimal Protocell ............................ 125
Steen Rasmussen, James Bailey, James Boncella, Liaohai
Chen, Gavin Collis, Stirling Colgate, Michael DeClue,
Harold Fellermann, Goran Goranovic, Yi Jiang, Chad
Knutson, Pierre-Alain Monnard, Fouzi Mouffouk, Peter
E. Nielsen, Anjana Sen, Andy Shreve, Arvydas Tamulis,
Bryan Travis, Pawel Weronski, William H. Woodruff,
Jinsuo Zhang, Xin Zhou, and Hans Ziock
7 Population Analysis of Liposomes with Protein Synthesis
and a Cascading Genetic Network ............................ 157
Takeshi Sunami, Kanetomo Sato, Keitaro Ishikawa, and
Tetsuya Yomo
8 Constructive Approach to Protocells: Theory and
Experiments ................................................ 169
Kunihiko Kaneko
9 Origin of Life and Lattice Artificial Chemistry ............ 197
Naoaki Ono, Duraid Madina, and Takashi Ikegami
10 Models of Protocell Replication ............................ 213
Ricard V. Solé, Javier Macia, Harold Fellermann,
Andreea Munteanu, Josep Sardanyés, and Sergi Valverde
11 Compositional Lipid Protocells: Reproduction without
Polynucleotides ............................................ 233
Doron Lancet and Barak Shenhav
12 Evolutionary Microfluidic Complementation Toward
Artificial Cells ........................................... 253
John S. McCaskill
III Components ................................................ 295
13 Self-Replication and Autocatalysis ......................... 299
Volker Patzke and Günter von Kiedrowski
14 Replicator Dynamics in Protocells .......................... 317
Peter F. Stadler and Bärbel M.R. Stadler
15 Peptide Nucleic Acids as Prebiotic and Abiotic Genetic
Material ................................................... 337
Peter E. Nielsen
16 The Core of a Minimal Gene Set: Insights from Natural
Reduced Genomes ............................................ 347
Toni Gabaldón, Rosario Gil, Juli Peretó, Amparo
Latorre, and Andrés Moya
17 Parasitism and Protocells: Tragedy of the Molecular
Commons .................................................... 367
Jeffrey J. Tabor, Matthew Levy, Zachary Booth Simpson,
and Andrew D. Ellington
18 Forming the Essential Template for Life: The Physics of
Lipid Self-Assembly ........................................ 385
Ole G. Mouritsen and Ask F. Jakobsen
19 Numerical Methods for Protocell Simulations ................ 407
Yi Jiang, Bryan Travis, Chad Knutson, Jinsuo Zhang,
and Pawel Weronski
20 Core Metabolism as a Self-Organized System ................. 433
Eric Smith, Harold J. Morowitz, and Shelley D. Copley
21 Energetics, Energy Flow, and Scaling in Life ............... 461
William H. Woodruff
IV Broader Context ........................................... 475
22 Gánti's Chemoton Model and Life Criteria ................... 481
James Griesemer and Eörs Szathmáry
23 Viral Individuality and Limitations of the Life Concept .... 513
David C. Krakauer and Paolo Zanotto
24 Nonlinear Chemical Dynamics and the Origin of Life: The
Inorganic-Physical Chemist Point of View ................... 537
Jerzy Maselko and Maciej Maselko
25 Early Ancestors of Existing Cells .......................... 563
Andrew Pohorille
26 Prebiotic Chemistry, the Primordial Replicator, and
Modern Protocells .......................................... 583
Henderson James Cleaves II
27 Cell-like Entities: Scientific Challenges and Future
Applications ............................................... 615
John M. Frazier, Nancy Kelley-Loughnane, Sandra Trott,
Oleg Paliy, Mauricio Rodriguez Rodriguez, Leamon
Viveros, and Melanie Tomczak
28 Social and Ethical Issues Concerning Protocells ............ 641
Mark A. Bedau and Emily C. Parke
Glossary ...................................................... 655
About the Authors ............................................. 667
Index ......................................................... 679
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